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KMID : 0545119970070050318
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 5 p.318 ~ p.322
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Optimal Resolution of L-Carnitine from Racemic DL-Carnitine by Enterobacter sp. Assimilating D-Carnitine
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Hwang Ki-Chul
Bang Won-Gi
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Abstract
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In order to isolate a microorganism having preferential degradation of D-carnitine from DL-carnitine, a bacterium assimilating D-carnitine as a sole carbon and energy source was isolated from soil by enrichment culture and partially identified as Enterobacter sp. Also, a mutant having lessened L-carnitine decomposition rates was selected with nitrosoguanidine mutagenesis, which led to decrease the specific activities of carnitine dehydrogenase (7.6-fold) and β-hydroxybutyrate dehydrogenase (9.5-fold) as compared to the wild strain. Meanwhile, optimal culture conditions for optical resolution of DL-carnitine were investigated. Under optimal conditions, 3.53 g/ℓ L-carnitine was obtained from 20 g/ℓ DL-carnitine, which corresponded to 35.3% L-carnitine yield and 97.9% optical purity.
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